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KMID : 0378019610040030097
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New Medical Journal
1961 Volume.4 No. 3 p.97 ~ p.112
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Experimental Study of the Influence of Thyroid Gland which Affects the Allergic Changes of Spinal Cord
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Abstract
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Numerous works were publicized, in the past, concerning tissue allergy since Maurice Arthus in 1903. The purpose of this-study is to investigate the allergic changes of the spinal cord which might be influenced by the presence or absence of thyroid gland.
The animal used for this study is normal white rabbit, weighing approximately 2 kg. Horse serum is selected for antigen. For sensitization, ice of horse serum per kg of body weight is injected subcutaneously, daily for 4 days and an interval of 15 days thereafter, elapsed. To induce allergic changes in spinal cord, 0. 1 cc of horse serum per kg of body weight is injected into the cord, via 7th intervertebral space of, thoracic spine. After the injection into spinal cord, the rabbit is killed with bloodletting method in 3, 6, 12, 24, 48 and 72 hours and 1 and 2 weeks respectively and the spinal cord is examined macroscopically and histologically. As thyroid substance, thyroxin, 0.2gm per kg is used per oral route.
The experimental animal is grouped into 9 categories as follows;
I) Non-sensitizing control group
1) Sensitized control group
M Sensitized, thyroidectomized group
]y) Sensitized group in which thyroid substance is administered
y) Sensitized group in which thyroid gland is transplanted
~J) Sensitized group in which thyroid gland is transplanted after thyroidectomy
yp) Sensitized group in which thyroid substance is given after thyroidectomy
VM) The group in which spinal cord is examined after thyroidectomy
a ) The group in which spinal cord is examined after administration of thyroid substance.
In Group I, only mild changes both in leptomeninges and cord per se are observed. In relatively early stage, congestion, hemorrhage, cloudy swelling, inflammatory cell reaction and vasoporosis, all in mild degree, are observed. These, however, all dissipated within 24 hours. Mild gliosis is seen in 48 hours and disappeared in 72 ohurs.
In Group II, much severe reaction in comparison with Group. I is noted. Edema, cloudy swelling and perivascular hematogenous cell cuffing are seen in moderately severe degree within 24 hours. Glial proliferation and subendothelial connective tissue proliferation are detected in comparatively late stage and become moderately severe and persisted up to 2 weeks after the reinjection.
In Group III, cloudy swelling and demyelinization are worse than sensitized control group. However, perivascular hematogenous cell cuffing, glial proliferation and subendothelial connective tissue proliferation are milder than Group II,
In Group IV, inflammatory cell reaction, perivascular heatogenous cell cuffing, glial prolifaration and subendothelial connective tissue proliferation appear to be severer than Group II. However, cloudy swelling, hyaline degeneration, demyelinization, softening and vasoporosis are seen to be milder. In other words, generally, in this group, inflammatory changes are severer and degenerative changes are milder than Group II.
In Group V, all pathologic changes are rather similar to those of Group IV though somewhat milder.
In Groups VI & VII, both changes are similar to those of Group 11.
In Groups VIII & IX. no apparent changes are detected in cords.
In summarizing, 1) Cardinal features in the allergic changes of spinal cord are cloudy swelling, hyaline degeneration, softening, demyelinization, congestion, vasoporosis, inflammatory cell reaction, perivascular hematogenous cell cuffing, filial proliferation and subendothelial connective tissue proliferation.
2) Extirpation of thyroid gland from the body suppresses the inflammatory reactions. On the contrary, administration of thyroid substance augments the reactions.
3) Administration of thyroid substance ameliorates the degenerative changes.
4) Normal spinal cord is not affected by either thyroidectomy or administration of thyroid substance.
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